Anti-Phospho-STAT2 (Tyr690) Polyclonal Antibody is a Rabbit antibody targeting Phospho-STAT2 (Tyr690). Anti-Phospho-STAT2 (Tyr690) Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Mouse (predicted:Human,Rat,Dog,Horse)

1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Stat2 (Tyr690)) Polyclonal Antibody, Unconjugated (TMAB-01506) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-Stat2 (Tyr690)(TMAB-01506) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 105 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Stat2 around the phosphorylation site of Tyr690

Human

STAT2

Signal transducer and activator of transcription 2

STATs,Other factors,SARS Coronavirus,STATs

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.