Anti-Phospho-SNCA (Ser129) Antibody (6F53) is an antibody targeting Phospho-SNCA (Ser129). Anti-Phospho-SNCA (Ser129) Antibody (6F53) can be used in ELISA, IHC, IF.

Rabbit IgG

6F53

Human

1. IHC image of TMAH-00968 diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
2. Immunofluorescence staining of Hela cells(treated with 100mM EGF for 20min) with TMAH-00968 at 1:206,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ELISA, IHC, IF

IHC:1:50-1:200; IF:1:20-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human Phospho-SNCA (S129)

Human

6622

Neuroscience