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Anti-Phospho-RUNX1 (Ser435) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-RUNX1 (Ser435)。Anti-Phospho-RUNX1 (Ser435) Polyclonal Antibody 可用于 WB。
Anti-Phospho-RUNX1 (Ser435) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-RUNX1 (Ser435)。Anti-Phospho-RUNX1 (Ser435) Polyclonal Antibody 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,230 | 5日内发货 |
产品描述 | Anti-Phospho-RUNX1 (Ser435) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RUNX1 (Ser435). Anti-Phospho-RUNX1 (Ser435) Polyclonal Antibody can be used in WB. |
别名 | RUNX1 (p-Ser435), RUNX1 (p-S435), p-RUNX1 (Ser435), p-RUNX1 (S435) |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody TMAC-00177. The lane on the left is treated with antigen-specific peptide. |
应用 | |
推荐剂量 | WB: 1:500-1000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of Serine 435( S-N-S(p)-P-T) derived from Human AML1 |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 53 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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