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Anti-Phospho-RPS6KA1 (S380) Antibody (2P405) 是一种 Rabbit 抗体,靶向 Phospho-RPS6KA1 (S380)。Anti-Phospho-RPS6KA1 (S380) Antibody (2P405) 可用于 WB,ICC/IF,IP。
Anti-Phospho-RPS6KA1 (S380) Antibody (2P405) 是一种 Rabbit 抗体,靶向 Phospho-RPS6KA1 (S380)。Anti-Phospho-RPS6KA1 (S380) Antibody (2P405) 可用于 WB,ICC/IF,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,480 | 5日内发货 |
产品描述 | Anti-Phospho-RPS6KA1 (Ser380) Antibody (2P405) 是一种 Rabbit 抗体,靶向 Phospho-RPS6KA1 (Ser380)。Anti-Phospho-RPS6KA1 (Ser380) Antibody (2P405) 可用于 WB,ICC/IF,IP。 |
别名 | S6K-alpha-1, S6K alpha 1, RSK1p90, RSK1, RSK 1 p90, RSK 1, RSK, RPS6KA1 (p-Ser380), RPS6KA1 (p-S380), rps6ka, RPS6K1 alpha, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase 90kD polypeptide 1, ribosomal protein S6 kinase, p-RPS6KA1 (Ser380), p-RPS6KA1 (S380), pp90RSK1, polypeptide 1, Phospho-RPS6KA1 (S380), p90S6K, p90RSK1, p90 RSK1, OTTHUMP00000004113, Mitogen-activated protein kinase-activated protein kinase 1A, MGC79981, MAPKAPK-1a, MAPKAPK1A, MAPKAP kinase 1a, MAP kinase activated protein kinase 1a, KS6A1_HUMAN, HU1, HU 1, EC 2.7.11.1, dJ590P13.1, 90kD, 90 kDa ribosomal protein S6 kinase 1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Phospho-RSK1 (S380) on A431 cell lysates using anti-Phospho-RSK1 (S380) antibody at 1/1,000 dilution. 2. ICC staining Phospho-RSK1 (S380) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Phospho-RSK1 (S380) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Phospho-RSK1 (S380) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, are important signaling intermediates that mediate responses to a broad range of ligand-activated receptor tyrosine kinases. It has been established that Rsk-3 is not activated by MAP kinase in vitro, unlike Rsk-1 and Rsk-2. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. The Rsk family amino-terminal kinase domain is phosphorylated on Ser 227 by 3-phosphoinositide-dependent protein kinase-1 (PDK1), which increases the kinase activity of Rsk. In the carboxy-terminal kinase domain, Rsk-1 and Rsk-2 are autophosphorylated on Ser 380 and Ser 386, respectively, which mediates the docking of PDK1 to Rsk in order to promote phosphorylation of substrates, such as histone H3. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human RSK1 around the phosphorylation site of Ser380 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 90 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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