购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空
前往下单页继续购物
顶部生物活性储存和溶解信息计算器

Anti-Phospho-RELB (Ser276) Polyclonal Antibody

Rating icon 还可以
Anti-Phospho-RELB (Ser276) Polyclonal Antibody
产品编号 TMAC-02836
别名 RELB (p-Ser276), RELB (p-S276), p-RELB (Ser276), p-RELB (S276)

Anti-Phospho-RELB (Ser276) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-RELB (Ser276)。Anti-Phospho-RELB (Ser276) Polyclonal Antibody 可用于 WB,IHC,IF。

Anti-Phospho-RELB (Ser276) Polyclonal Antibody

Anti-Phospho-RELB (Ser276) Polyclonal Antibody

Rating icon 还可以
Anti-Phospho-RELB (Ser276) Polyclonal Antibody
产品编号 TMAC-02836 别名 RELB (p-Ser276), RELB (p-S276), p-RELB (Ser276), p-RELB (S276)

Anti-Phospho-RELB (Ser276) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-RELB (Ser276)。Anti-Phospho-RELB (Ser276) Polyclonal Antibody 可用于 WB,IHC,IF。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,230
5日内发货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
查看更多
联系我们获取更多批次信息
资源下载
产品操作手册

产品介绍

生物活性
产品描述
Anti-Phospho-RELB (Ser276) Polyclonal Antibody is a Rabbit antibody targeting Phospho-RELB (Ser276). Anti-Phospho-RELB (Ser276) Polyclonal Antibody can be used in WB,IHC,IF.
别名RELB (p-Ser276), RELB (p-S276), p-RELB (Ser276), p-RELB (S276)
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Immunofluorescence staining of methanol-fixed Hela cells using NFκB-p65 (Phospho-Ser276) Antibody TMAC-02836.
2. Western blot analysis of extract from Hela cells using NF-κB p65 (phospho-Ser276) antibody TMAC-02836. Lane 1: The antibody is not preincubated with blocking peptides. Lane 2: The antibody is preincubated with non-phospho peptide blocking peptides. Lane 3: The antibody is preincubated with phospho peptide blocking peptides.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) Antibody TMAC-02836 (left) or the same antibody preincubated with blocking peptide (right).
4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody TMAC-02836.
5. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody TMAC-02836.
6. Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NFκB-p65 (Phospho-Ser276) Antibody TMAC-02836.
应用
WB
IHC
IF
推荐剂量
WB: 1:500-1000; IF: 1:100-200; IHC: 1:50-100
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
性状Liquid
缓冲液Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
Peptide sequence around phosphorylation site of serine 276(R-P-S(p)-D-R) derived from Human NFκB-p65
抗原种属
human
Uniprot ID
Q04206
化学信息
分子量Actual: 65 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器

关键词

RELB (pSer276)RELB (pS276)RELB (phospho-Ser276)RELB (phospho-S276)pRELB (Ser276)pRELB (S276)

评论列表

4个月前
5.0
Rating icon 很棒

评论内容

233
Related Tags: buy Anti-Phospho-RELB (Ser276) Polyclonal Antibody | purchase Anti-Phospho-RELB (Ser276) Polyclonal Antibody | Anti-Phospho-RELB (Ser276) Polyclonal Antibody cost | order Anti-Phospho-RELB (Ser276) Polyclonal Antibody | Anti-Phospho-RELB (Ser276) Polyclonal Antibody molecular weight