Anti-Phospho-RB1 (Ser780) Antibody (1X20) is an antibody targeting Phospho-RB1 (Ser780). Anti-Phospho-RB1 (Ser780) Antibody (1X20) can be used in ELISA, IHC, IF, IP.

Rabbit IgG

1X20

Human

1. IHC image of TMAH-00956 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
2. Immunofluorescence staining of K562 cells with TMAH-00956 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
3. Immunoprecipitating Phospho-RB1 in Hela whole cell lysate
Lane 1: Rabbit control IgG(1μg)instead of TMAH-00956 in Hela whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: TMAH-00956(3μg)+ Hela whole cell lysate(1mg)
Lane 3: Hela whole cell lysate (20μg)

ELISA, IHC, IF, IP

IHC:1:50-1:200; IF:1:20-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human Phospho-RB1 (S780)

Human

5925

Cell Biology