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Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-PRKCA (Thr197)。Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody 可用于 WB,IHC,IF。
Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-PRKCA (Thr197)。Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody 可用于 WB,IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,245 | 5日内发货 |
产品描述 | Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PRKCA (Thr197). Anti-Phospho-PRKCA (Thr197) Polyclonal Antibody can be used in WB,IHC,IF. |
别名 | Protein kinase C zeta, Protein kinase C gamma, Protein kinase C epsilon, Protein kinase C delta, Protein kinase C beta, Protein kinase C alpha, Protein kinase C, PRKCZ, PRKCG, PRKCE, PRKCD, PRKCB2, PRKCB1, PRKCB, PRKCA (p-Thr197), PRKCA (p-T197), PRKCA, p-PRKCA (Thr197), p-PRKCA (T197), PKCG, PKCE, PKCD, PKCB, PKC-alpha, PKC-A, PKCA, PKC2, PKC zeta, PKC gamma, PKC epsilon, PKC delta, PKC beta, PKC alpha, Phospho-PRKCA (T197), KPCA_HUMAN |
Ig Type | IgG |
交叉反应 | Human,Mouse |
验证活性 | 1. Western blot analysis of extracts from mouse brain cells using PKA α/β CAT (Phospho-Thr197) Antibody TMAC-03308.The lane on the right is treated with the antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA α/β CAT (Phospho-Thr197) antibody TMAC-03308 (left) or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed A549 cells using PKA α/β CAT (Phospho-Thr197) Antibody TMAC-03308. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-100; IF: 1:100-200 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of threonine 197(T-W-T(p)-L-C) derived from Human PKA α/β CAT |
抗原种属 | human |
分子量 | Actual: 40 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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