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Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody
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产品编号 TMAB-01480
Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-PIK3CA (Tyr317)。Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody
Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody
还可以产品编号 TMAB-01480
Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-PIK3CA (Tyr317)。Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PIK3CA (Tyr317). Anti-Phospho-PIK3CA (Tyr317) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-PI3KCA (Tyr317)) Polyclonal Antibody, Unconjugated (TMAB-01480) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 μg Lane 3: Jurkat (Human) Cell Lysate at 30 μg Lane 4: MCF-7 (Human) Cell Lysate at 30 μg Lane 5: A431 (Human) Cell Lysate at 30 μg Lane 6: Siha (Human) Cell Lysate at 30 μg Primary: Anti-phospho-PI3KCA (Tyr317) (TMAB-01480) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110/120 kDa Observed band size: 110 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdInsP2. Four isoforms of p110 has been found; alpha, beta, gamma, and the delta subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach. |
| 免疫原 | KLH conjugated Synthesised phosphopeptide: human PI3KCA around the phosphorylation site of Tyr317 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Signal transducers,Signal transduction,TLR Signaling,Lipid Kinases |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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