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Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) 是一种抗体,靶向 Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602)。Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) 可用于 ELISA, WB, IF。
Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) 是一种抗体,靶向 Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602)。Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) 可用于 ELISA, WB, IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,300 | 5日内发货 | |
100 μL | ¥ 2,180 | 5日内发货 |
产品描述 | Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) is an antibody targeting Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602). Anti-Phospho-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602) Antibody (4J12) can be used in ELISA, WB, IF. |
别名 | p-PAK4/PAK5/PAK6 (Ser474/Ser560/Ser602), p-PAK4/PAK5/PAK6 (S474/S560/S602), Phospho-PAK4/PAK5/PAK6 (S474/S560/S602), PAK4/PAK5/PAK6 (p-Ser474/Ser560/Ser602), PAK4/PAK5/PAK6 (p-S474/S560/S602) |
Ig Type | Rabbit IgG |
克隆号 | 4J12 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not) -All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 64,75,80 KDa -Observed band size: 75,80 KDa 2. Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with TMAH-00938 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
应用 | ELISA, WB, IF |
推荐剂量 | WB:1:500-1:5000; IF:1:20-1:200. |
抗体种类 | Monoclonal |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) |
抗原种属 | Human |
Uniprot ID | |
研究领域 | Cell Biology |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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