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Anti-Phospho-p53 (Ser15) Antibody (5X80)
还可以产品编号 TMAY-01535
Anti-Phospho-p53 (Ser15) Antibody (5X80) 是一种 Rabbit 抗体，靶向 Phospho-p53 (Ser15)。Anti-Phospho-p53 (Ser15) Antibody (5X80) 可用于 WB。
Anti-Phospho-p53 (Ser15) Antibody (5X80)
Anti-Phospho-p53 (Ser15) Antibody (5X80)
还可以产品编号 TMAY-01535
Anti-Phospho-p53 (Ser15) Antibody (5X80) 是一种 Rabbit 抗体，靶向 Phospho-p53 (Ser15)。Anti-Phospho-p53 (Ser15) Antibody (5X80) 可用于 WB。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-p53 (Ser15) Antibody (5X80) is a Rabbit antibody targeting Phospho-p53 (Ser15). Anti-Phospho-p53 (Ser15) Antibody (5X80) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 5X80 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of extracts from MCF-7, untreated (line A); treated with Doxorubicin (1 μM, 8 h), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p53 (Ser15) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) 2. Western blot analysis of extracts from MCF-7, untreated (-) or treated with Doxorubicin (1 μM, 8 h) (+), using Phospho-p53 (Ser15) Antibody, Rabbit Mab at 1:2000 dilution (upper) or Anti-p53 Antibody, Rabbit Monoclonal (middle) at 1:200 dilution or Beta-Tubulin Loading Control Antibody, Mouse Mab at 1:20000 dilution. |
| 应用 | WB |
| 推荐剂量 | WB: 1:1000-1:5000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser15 of the Human Phospho-p53. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitin ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic phosphopeptide corresponding to residues around Ser15 of the Human Phospho-p53 |
| 抗原种属 | Human |
| 研究领域 | Apoptosis Transcription Factors and Regulators, Cancer Drug Targets, Tumor Suppressors |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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