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Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703)

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产品编号 TMAY-01674
别名 p65, NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3

Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 是一种 Rabbit 抗体,靶向 Phospho-NF-κB p65 (Ser536)。Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 可用于 WB。

Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703)

Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703)

Rating icon 还可以
产品编号 TMAY-01674 别名 p65, NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3

Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 是一种 Rabbit 抗体,靶向 Phospho-NF-κB p65 (Ser536)。Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 可用于 WB。

规格价格库存数量
20 μL
¥ 689
5日内发货
100 μL
¥ 1,994
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) is a Rabbit antibody targeting Phospho-NF-κB p65 (Ser536). Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) can be used in WB.
别名p65, NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3
Ig Type
Monoclonal Rabbit IgG
克隆号
5Y703
交叉反应
Human;
Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus
验证活性
1. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min) (line B); treated with Calyculin A and λ-phosphatase (line C) using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution.

2. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution (upper) or Beta-Tubulin Loading Control Antibody, Mouse MAb at 1:20000 dilution (lower).

3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution. (Validation Experiment)

4. Western blot analysis of extracts from serum-starved NIH/3T3, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment)
应用
WB
推荐剂量
WB: 1:2000-1:20000
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-κB p65.
纯化方式Protein A
性状Liquid
缓冲液10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
研究背景RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-κB p65
抗原种属
Human
研究领域
Apoptosis Transcription Factors and Regulators
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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