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Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 是一种 Rabbit 抗体,靶向 Phospho-NF-κB p65 (Ser536)。Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 可用于 WB。
Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 是一种 Rabbit 抗体,靶向 Phospho-NF-κB p65 (Ser536)。Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
20 μL | ¥ 689 | 5日内发货 | |
100 μL | ¥ 1,994 | 5日内发货 |
产品描述 | Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) is a Rabbit antibody targeting Phospho-NF-κB p65 (Ser536). Anti-Phospho-NF-κB p65 (Ser536) Antibody (5Y703) can be used in WB. |
别名 | p65, NF-κB, NFκB, NF-κ B, NFKB3, NF-kB, NFkB, NF-kappaB, NFkappaB, NF-kappa B, NF-k B, CMCU, AIF3BL3 |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 5Y703 |
交叉反应 | Human;
Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus |
验证活性 | 1. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min) (line B); treated with Calyculin A and λ-phosphatase (line C) using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution. 2. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution (upper) or Beta-Tubulin Loading Control Antibody, Mouse MAb at 1:20000 dilution (lower). 3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (200 nM, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:5000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved NIH/3T3, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-NF-κB p65 (Ser536) Antibody, Rabbit MAb at 1:1000 dilution. (Validation Experiment) |
应用 | WB |
推荐剂量 | WB: 1:2000-1:20000 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-κB p65. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
研究背景 | RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation. |
偶联 | Unconjugated |
免疫原 | A synthetic phosphopeptide corresponding to residues around Ser536 of the Human NF-κB p65 |
抗原种属 | Human |
研究领域 | Apoptosis Transcription Factors and Regulators |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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