Anti-Phospho-MLKL (Ser345) Antibody (2S387) is a Rabbit antibody targeting Phospho-MLKL (Ser345). Anti-Phospho-MLKL (Ser345) Antibody (2S387) can be used in IP,WB.

IgG

2S387

Mouse (predicted:Human)

1. Western blot analysis of Phospho-MLKL (S345) on L929 cell lysates.
Lane 1: L929 cells, whole cell lysate, 10 μg/lane.
Lane 2: L929 cells were treated with 20 uM Z-VAD for 30 minutes, then added 20 ng/ml mTNF-alpha and 100 nM SM-164 for 4 hours, whole cell lysates, 10 μg/lane.
2. 4% Paraformaldehyde-fixed L-929 (treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic, and 20 µM z-VAD for 8 h) (M) cell; Triton X-100 at RT for 20 min; Antibody incubation with (phospho-MLKL (Ser345)) monoclonal Antibody, unconjugated (TMAB-01454) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; IP: 1:10-50

Monoclonal

A synthesized peptide: mouse Mlkl around the phosphorylation site of S345

Mouse

MLKL

Mixed lineage kinase domain-like protein

Necroptosis,SARS Coronavirus,Other Kinases

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity.