Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MKK3/6 (Ser218, 207). Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (TMAB-01453) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-MKK3 (Ser218) + MKK6 (Ser207) (TMAB-01453) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa
3. Sample:
Muscle (Rat) Lysate at 40 μg
Primary: Anti-Phospho-MKK3 (Ser218) + MKK6 (Ser207) (TMAB-01453) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa
4. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (TMAB-01453) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (TMAB-01453) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human MKK3 around the phosphorylation site of Ser218

Human

MAPK pathway,Other factors,TLR Signaling,MAPK Pathway,Tyrosine Kinases