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Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody
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产品编号 TMAB-01450
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-LATS1/2 (Thr1079, 1041)。Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody
还可以产品编号 TMAB-01450
Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-LATS1/2 (Thr1079, 1041)。Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,970 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody is a Rabbit antibody targeting Phospho-LATS1/2 (Thr1079, 1041). Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Pig,Cow,Horse) |
| 验证活性 | 1. phosphopeptide non phosphopeptide 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-LATS1+LATS2 (Thr1079 +Thr1041)) Polyclonal Antibody, Unconjugated (TMAB-01450) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (TMAB-01450) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa 4. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (TMAB-01450) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq]. |
| 免疫原 | KLH conjugated synthesised phosphopeptide: human LATS1 around the phosphorylation site of Thr1079 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Tumor suppressors,Kinases,Cytokinesis,Cdk Regulators |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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