Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody is a Rabbit antibody targeting Phospho-LATS1/2 (Thr1079, 1041). Anti-Phospho-LATS1/2 (Thr1079, 1041) Polyclonal Antibody can be used in ELISA,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rabbit (predicted:Rat,Chicken,Dog,Pig,Cow,Horse)

1. phosphopeptide
non phosphopeptide
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-LATS1+LATS2 (Thr1079 +Thr1041)) Polyclonal Antibody, Unconjugated (TMAB-01450) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (TMAB-01450) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
4. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-phospho-LATS1+LATS2 (Thr1079 +Thr1041) (TMAB-01450) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthesised phosphopeptide: human LATS1 around the phosphorylation site of Thr1079

Human

LATS1

Serine/threonine-protein kinase LATS1

Tumor suppressors,Kinases,Cytokinesis,Cdk Regulators

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.