- 全部删除
- 您的购物车当前为空
Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-JAK1 (Tyr1022)。Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody 可用于 WB,IHC,IF。
Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-JAK1 (Tyr1022)。Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody 可用于 WB,IHC,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,230 | 5日内发货 |
产品描述 | Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK1 (Tyr1022). Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody can be used in WB,IHC,IF. |
别名 | p-JAK1 (Y1022), p-JAK1 (Tyr1022), JAK1 (p-Y1022), JAK1 (p-Tyr1022) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western Blot analysis of various cells using Phospho-JAK1 (Y1034) Polyclonal Antibody diluted at 1:500. 2. Western blot analysis of lysates from A549 cells, using JAK1 (Phospho-Tyr1034) Antibody. The lane on the right is blocked with the phospho peptide. 3. Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2,Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20min). 3,Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only. 4. Immunofluorescence analysis of mouse-liver tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2,Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B. |
应用 | |
推荐剂量 | WB: 1:200-1000; IHC: 1:100-300; IF: 1:50-200 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
研究背景 | Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of tyrosine 1034 (K-E-Y(p)-Y-T) derived from Human JAK1 |
抗原种属 | human |
Uniprot ID |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
评论内容