Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRF7 (Ser471, 472). Anti-Phospho-IRF7 (Ser471, 472) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse)

1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) polyclonal Antibody, Unconjugated (TMAB-01437) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01437) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (TMAB-01437) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Thymus tissue lysates
Lane 3: Mouse Blood cell lysates
Lane 4: Mouse Lung tissue lysates
Lane 5: Rat Spleen tissue lysates
Lane 6: Rat Liver tissue lysates
Lane 7: Human Jurkat cell lysates
Lane 8: Human Raji cell lysates
Primary: Anti-Phospho-IRF7 (Ser471 + Ser472) (TMAB-01437) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 70,55 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human IRF7 around the phosphorylation site of Ser471/472

Human

Helix-Turn-Helix,Pol II Transcription,Antiviral Signaling,Interferons,TLR Signaling,Host Virus Interaction,SARS Coronavirus