Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser36). Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse (predicted:Rat,Pig,Cow,Rabbit,Sheep)

1. Sample:
Spleen (Mouse) Lysate at 40 μg
BV2 (Mouse) Lysate at 30 μg
Primary: Anti-phospho-IKB alpha (Ser36) (TMAB-01427) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKB alpha (Ser36)) Polyclonal Antibody, Unconjugated (TMAB-01427) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-IKB alpha (Ser36)) polyclonal Antibody, Unconjugated (TMAB-01427) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human IKB alpha around the phosphorylation site of Ser36

Human

NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway