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Anti-Phospho-H2AFX (S139) Antibody (2B111) 是一种 Rabbit 抗体,靶向 Phospho-H2AFX (S139)。Anti-Phospho-H2AFX (S139) Antibody (2B111) 可用于 WB,IHC,ICC/IF,IP。
Anti-Phospho-H2AFX (S139) Antibody (2B111) 是一种 Rabbit 抗体,靶向 Phospho-H2AFX (S139)。Anti-Phospho-H2AFX (S139) Antibody (2B111) 可用于 WB,IHC,ICC/IF,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-Phospho-H2AFX (Ser139) Antibody (2B111) 是一种 Rabbit 抗体,靶向 Phospho-H2AFX (Ser139)。Anti-Phospho-H2AFX (Ser139) Antibody (2B111) 可用于 WB,IHC,ICC/IF,IP。 |
别名 | p-H2AFX (Ser139), p-H2AFX (S139), H2AFX (p-Ser139), H2AFX (p-S139) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Phospho-Histone H2A.X (S139) on HepG2 cell lysates using anti-Phospho-Histone H2A.X (S139) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 cell lysate treated with etoposide, Lane 2: HepG2 cell lysate untreated. 2. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Phospho-Histone H2A.X (S139) antibody. Counter stained with hematoxylin. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:100-200; ICC/IF: 1:50-200; IP: 1:20-50 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Histone H2A.X is a member of the Histone H2A family, which is involved in nucleosomal organization of chromatin. The H2AFX gene is located in close proximity to the Porphobilinogen deaminase (PBG-D) gene in both mouse and human, and maps to chromosome 9 and 11q23, respectively. H2A.X differs from the other members of the H2A family by the presence of a highly conserved C-terminal motif. It is rapidly phosphorylated in response to ionizing radiation and plays an important role in the recognition and repair of DNA double stranded breaks. The phosphorylated form of H2A.X, designated ?-H2A.X, forms nuclear foci at the heavy chain constant region of cells involved in class switch recombination (CSR), a region-specific DNA reaction that replaces one immunoglobulin heavy chain constant region gene with another. The phosphorylated ?-H2A.X is also thought to initiate subsequent repair factors, including Rad50, Rad51 and BRCA1. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human Histone H2A.X around the phosphorylation site of Ser139 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 15 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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