Anti-Phospho-FAK (Tyr397) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FAK (Tyr397). Anti-Phospho-FAK (Tyr397) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Rabbit)

1. Sample:
Thymus (Mouse) Lysate at 40 μg
Primary: Anti-p-FAK (Tyr397) (TMAB-01411) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 116 kDa
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P-FAK (Tyr397)) Polyclonal Antibody, Unconjugated (TMAB-01411) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 μg
Lane 2: Testis (Mouse) Lysate at 40 μg
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 4: Cerebrum (Rat) Lysate at 40 μg
Lane 5: Adrenal gland (Rat) Lysate at 40 μg
Lane 6: Testis (Rat) Lysate at 40 μg
Lane 7: 293T (Human) Cell Lysate at 30 μg
Lane 8: A431 (Human) Cell Lysate at 30 μg
Lane 9: Huvec (Human) Cell Lysate at 30 μg
Lane 10: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 11: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Phospho-FAK (Tyr397) (TMAB-01411) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 125 kDa
4. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FAK (Tyr397)) Polyclonal Antibody, Unconjugated (TMAB-01411) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-FAK (Tyr397)) polyclonal Antibody, Unconjugated (TMAB-01411) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50; IF: 1:100-500

Polyclonal

KLH cunjugated Synthesised phosphopeptide: human FAK around the phosphorylation site of Tyr397

Human

FAK family,Integrins,Focal Adhesions,FAK / PYK