Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ERK5 (Ser496). Anti-Phospho-ERK5 (Ser496) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human (predicted:Mouse,Rat)

1. Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 μg
Lane 2: Hcclm3 (Human) Cell Lysate at 30 μg
Primary:
Anti-phospho-ERK5 (Ser496) (TMAB-01410) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 115 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-ERK5 (Ser496)) Polyclonal Antibody, Unconjugated (TMAB-01410) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-ERK5 (Ser496)) polyclonal Antibody, Unconjugated (TMAB-01410) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human ERK5 around the phosphorylation site of Ser496

Human

Neural Signal Transduction,Kinases,MAPK Pathway,Other Kinases