Anti-Phospho-EphA2 (Tyr588, 596) Polyclonal Antibody is a Rabbit antibody targeting Phospho-EphA2 (Tyr588, 596). Anti-Phospho-EphA2 (Tyr588, 596) Polyclonal Antibody can be used in WB,IF.

IgG

Human,Mouse,Rat

1. Western blot analysis of EPHA2 (phospho-Tyr588/596) expression in A431, mouse brain whole cell lysates.
2. Immunofluorescent analysis of EPHA2 (phospho-Tyr588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

WB: 1:500-1000; IF:1:100-300

Polyclonal

Unconjugated

Phospho

KLH conjugated synthetic peptide: encompassing a sequence within the center region of human EPHA2

human