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Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-eNOS (Ser1177)。Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody 可用于 WB,IF。
Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-eNOS (Ser1177)。Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody 可用于 WB,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,235 | 5日内发货 |
产品描述 | Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody is a Rabbit antibody targeting Phospho-eNOS (Ser1177). Anti-Phospho-eNOS (Ser1177) Polyclonal Antibody can be used in WB,IF. |
别名 | p-eNOS (Ser1177), p-eNOS (S1177), eNOS (p-Ser1177), eNOS (p-S1177) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western Blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000. 2. Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using eNOS (Phospho-Ser1176) Antibody. The lane on the right is blocked with the phospho peptide. 3. Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. 4. Immunofluorescence analysis of Hela cell. 1,NOS3 (phospho Ser1177) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min. |
应用 | |
推荐剂量 | WB: 1:500-2000; IF: 1:200-1000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
性状 | Liquid |
缓冲液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
研究背景 | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009], |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | A synthesized phosphopeptide: human eNOS around the phosphorylation site of Ser1176 |
抗原种属 | human |
Uniprot ID |
分子量 | Theoretical: 130-140 kDa. |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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