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Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529)
还可以产品编号 TMAY-01717
Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) 是一种 Rabbit 抗体，靶向 Phospho-EGF Receptor (Thr669)。Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) 可用于 WB。
Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529)
Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529)
还可以产品编号 TMAY-01717
Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) 是一种 Rabbit 抗体，靶向 Phospho-EGF Receptor (Thr669)。Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) 可用于 WB。
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抗原信息
| 产品描述 | Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) is a Rabbit antibody targeting Phospho-EGF Receptor (Thr669). Anti-Phospho-EGF Receptor (Thr669) Antibody (8Z529) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8Z529 |
| 交叉反应 | Human; Predicted to React with:Species predicted to react based on 100% sequence homology: Mouse, Cynomolgus |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved Hela, untreated(line A) or treated with EGF (100ng/mL, 10min; +)(line B), using Phospho-EGF Receptor (Thr669) rabbit monoclonal Antibody at 1:2000 dilution (upper) or Anti-EGF Receptor Antibody, Rabbit Polyclonal at 1:1000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with EGF (100ng/mL, 10min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-EGF Receptor (Thr669) rabbit monoclonal Antibody at 1:2000 dilution. (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:2000-1:20000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Thr669) of Human EGF Receptor. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the residues around (Thr669) of Human EGF Receptor |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets, Receptor Tyrosine Kinases (RTKs) |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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