Anti-Phospho-eEF2 (Thr56) Antibody (6P325) is a Rabbit antibody targeting Phospho-eEF2 (Thr56). Anti-Phospho-eEF2 (Thr56) Antibody (6P325) can be used in WB.

Monoclonal Rabbit IgG

6P325

Human;

Predicted to React with: HumanSpecies predicted to react based on 100% sequence homology: Mouse, Rat, Bovine

1. Western blot analysis of extracts from serum-starved HepG2 , untreated(line A) or treated with forskolin(30μM, 30min)(line B), using Phospho-eEF2 (Thr56) rabbit monoclonal Antibody at 1:10000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower).

2. Western blot analysis of extracts from serum-starved HepG2 , untreated (line A); treated with forskolin(30μM, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-eEF2 (Thr56) rabbit monoclonal Antibody at 1:10000 dilution. (Validation Experiment)

3. Western blot analysis of extracts from serum-starved HepG2, untreated (line A); treated with forskolin(30μM, 30min; +) (line B); treated with forskolin and λ-phosphatase (line C) using Phospho-eEF2 (Thr56) rabbit monoclonal Antibody at 1:10000 dilution. (Validation Experiment)

WB

WB: 1:10000-1:100000

Monoclonal

Unconjugated

A synthetic peptide corresponding to the residues around (Thr56) of Human Phospho-eEF2

Human