Anti-Phospho-Crkl (Tyr207) Antibody (3X40) is a Rabbit antibody targeting Phospho-Crkl (Tyr207). Anti-Phospho-Crkl (Tyr207) Antibody (3X40) can be used in FCM, WB.

IgG

3X40

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with Phospho-Crkl (Tyr207) monoclonal antibody, unconjugated (TMAB-10544) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The K562 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Phospho-Crkl (Tyr207) antibody (TMAB-10544, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; WB=1:500-2000

Monoclonal

A synthesized peptide: human CRKL around the phosphorylation site of Y207

Human

CRKL

Crk-like protein

May mediate the transduction of intracellular signals.