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Anti-Phospho-Claudin-3 (Tyr219) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-Claudin-3 (Tyr219)。Anti-Phospho-Claudin-3 (Tyr219) Polyclonal Antibody 可用于 WB。
Anti-Phospho-Claudin-3 (Tyr219) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-Claudin-3 (Tyr219)。Anti-Phospho-Claudin-3 (Tyr219) Polyclonal Antibody 可用于 WB。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,240 | 5日内发货 |
产品描述 | Anti-Phospho-Claudin-3 (Tyr219) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Claudin-3 (Tyr219). Anti-Phospho-Claudin-3 (Tyr219) Polyclonal Antibody can be used in WB. |
别名 | p-Claudin-3 (Y219), p-Claudin-3 (Tyr219), Claudin-3 (p-Y219), Claudin-3 (p-Tyr219) |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of extracts from COLO cells treated with EGF using Claudin 3 (Phospho-Tyr219) Antibody TMAC-00886. The lane on the right is treated with the antigen-specific peptide. |
应用 | |
推荐剂量 | WB: 1:500-1000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of tyrosine 219 (R-K-D-Y(p)-V) derived from Human Claudin 3 |
抗原种属 | human |
Uniprot ID |
分子量 | Actual: 28 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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