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Anti-Phospho-Chk2 (Thr68) Antibody (7T986)
还可以产品编号 TMAY-01664
Anti-Phospho-Chk2 (Thr68) Antibody (7T986) 是一种 Rabbit 抗体，靶向 Phospho-Chk2 (Thr68)。Anti-Phospho-Chk2 (Thr68) Antibody (7T986) 可用于 WB。
Anti-Phospho-Chk2 (Thr68) Antibody (7T986)
Anti-Phospho-Chk2 (Thr68) Antibody (7T986)
还可以产品编号 TMAY-01664
Anti-Phospho-Chk2 (Thr68) Antibody (7T986) 是一种 Rabbit 抗体，靶向 Phospho-Chk2 (Thr68)。Anti-Phospho-Chk2 (Thr68) Antibody (7T986) 可用于 WB。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-Chk2 (Thr68) Antibody (7T986) is a Rabbit antibody targeting Phospho-Chk2 (Thr68). Anti-Phospho-Chk2 (Thr68) Antibody (7T986) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 7T986 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of extracts from HCT116, untreated (-) or treated with Etoposide (100 μM, 2 h) (+), using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb at 1:500 dilution (upper) or Anti-CHEK2 Antibody, Rabbit PAb (middle) at 1:1000 dilution or Beta-Tubulin Loading Control Antibody, Mouse Mab at 1:10000 dilution. 2. Western blot analysis of extracts from HCT116, untreated (line A); treated with Etoposide (100 μM, 2 h), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb at 1:500 dilution. (Validation Experiment) 3. Western blot analysis of extracts from HCT116, untreated (line A); treated with Etoposide (100 μM, 2 h) (line B); treated with Etoposide and λ-phosphatase (line C) using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb at 1:500 dilution. (Validation Experiment) 4. Western blot analysis of extracts from HCT116, treated with Etoposide (100μM, 2 h), using Phospho-Chk2 (Thr68) Antibody, Rabbit MAb and other brands’ antibodies (Company C) at dilution of 1:1000, 1:5000. (Validation Experiment) |
| 应用 | WB |
| 推荐剂量 | WB: 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Thr68 of the Human Chk2. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic phosphopeptide corresponding to residues around Thr68 of the Human Chk2 |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets, Tumor Suppressors |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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