Anti-Phospho-cdc25A (Ser76) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-cdc25A (Ser76). Anti-Phospho-cdc25A (Ser76) Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Cow,Horse,Rabbit)

1. Sample: Embryo (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-cdc25A (Ser76) (TMAB-10454) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
2. Sample:
Testis (Mouse) Lysate at 40 μg
MCF-7 (Human) Lysate at 30 μg
Primary: Anti-Phospho-cdc25A (Ser76) (TMAB-10454) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
3. Blank control (black line): HepG2.
Primary Antibody (green line): Rabbit Anti-Phospho-cdc25A (Ser76) antibody (TMAB-10454)
Dilution: 2 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthesised phosphopeptide: human cdc25A around the phosphorylation site of Ser76

Human

CDC25A

M-phase inducer phosphatase 1

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.