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Anti-Phospho-cdc2 (Tyr15) Antibody (2T187)
还可以产品编号 TMAY-01670
Anti-Phospho-cdc2 (Tyr15) Antibody (2T187) 是一种 Rabbit 抗体，靶向 Phospho-cdc2 (Tyr15)。Anti-Phospho-cdc2 (Tyr15) Antibody (2T187) 可用于 WB。
Anti-Phospho-cdc2 (Tyr15) Antibody (2T187)
Anti-Phospho-cdc2 (Tyr15) Antibody (2T187)
还可以产品编号 TMAY-01670
Anti-Phospho-cdc2 (Tyr15) Antibody (2T187) 是一种 Rabbit 抗体，靶向 Phospho-cdc2 (Tyr15)。Anti-Phospho-cdc2 (Tyr15) Antibody (2T187) 可用于 WB。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-cdc2 (Tyr15) Antibody (2T187) is a Rabbit antibody targeting Phospho-cdc2 (Tyr15). Anti-Phospho-cdc2 (Tyr15) Antibody (2T187) can be used in WB. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 2T187 |
| 交叉反应 | Human; Species predicted to react based on 100% sequence homology: Mouse, Rat, Cynomolgus, Bovin, Chick |
| 验证活性 | 1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with hydroxyurea (4mm, 24h; +), using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb at 1:10000 dilution (upper) or Anti-CDK1/CDC2 Antibody, Rabbit PAb at 1:2000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with hydroxyurea (4mm, 24h, +) (line B); treated with hydroxyurea and λ-phosphatase (line C) using Phospho-Cdc2 (Tyr15) Antibody, Rabbit MAb at 1:10000 dilution. |
| 应用 | WB |
| 推荐剂量 | WB: 1:5000-1:50000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Tyr15 of the Human cdc2. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| 研究背景 | CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic phosphopeptide corresponding to residues around Tyr15 of the Human cdc2 |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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