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Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody
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产品编号 TMAC-00116
别名 p-AKT1 (Thr308), p-AKT1 (T308), AKT1 (p-Thr308), AKT1 (p-T308)
Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-AKT1 (Thr308)。Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody 可用于 WB,IHC,IF。
Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody
Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody
还可以产品编号 TMAC-00116 别名 p-AKT1 (Thr308), p-AKT1 (T308), AKT1 (p-Thr308), AKT1 (p-T308)
Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Phospho-AKT1 (Thr308)。Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody 可用于 WB,IHC,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,240 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AKT1 (Thr308). Anti-Phospho-AKT1 (Thr308) Polyclonal Antibody can be used in WB,IHC,IF. |
| 别名 | p-AKT1 (Thr308), p-AKT1 (T308), AKT1 (p-Thr308), AKT1 (p-T308) |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of extracts from Hela cells untreated or treated with EGF, serum or both using Akt(Phospho-Thr308) Antibody TMAC-00116. 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody TMAC-00116 (left) or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt(Phospho-Thr308) Antibody TMAC-00116. 4. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody TMAC-00116. 5. Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt (Phospho-Thr308) Antibody TMAC-00116. |
| 应用 | |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Polyclonal Antibody |
| 纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 性状 | Liquid |
| 缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Phospho |
| 免疫原 | Peptide sequence around phosphorylation site of threonine 308 (M-K-T(p)-F-C) derived from Human Akt |
| 抗原种属 | human |
| Uniprot ID |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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