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Anti-Phospho-4EBP1 (Thr46) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-4EBP1 (Thr46)。Anti-Phospho-4EBP1 (Thr46) Polyclonal Antibody 可用于 WB,IHC。
Anti-Phospho-4EBP1 (Thr46) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-4EBP1 (Thr46)。Anti-Phospho-4EBP1 (Thr46) Polyclonal Antibody 可用于 WB,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,245 | 5日内发货 |
产品描述 | Anti-Phospho-4EBP1 (Thr46) Polyclonal Antibody is a Rabbit antibody targeting Phospho-4EBP1 (Thr46). Anti-Phospho-4EBP1 (Thr46) Polyclonal Antibody can be used in WB,IHC. |
别名 | p-4EBP1 (Thr46), p-4EBP1 (T46), 4EBP1 (p-Thr46), 4EBP1 (p-T46) |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of extracts from Hela cells using 4E-BP1 (Phospho-Thr46) Antibody TMAC-00022 and the same antibody preincubated with blocking peptide. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1(Phospho-Thr45) Antibody TMAC-00022(left) or the same antibody preincubated with blocking peptide(right). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-100 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
构建方式 | Polyclonal Antibody |
纯化方式 | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
性状 | Liquid |
缓冲液 | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
偶联 | Unconjugated |
其他可选修饰符 | Phospho |
免疫原 | Peptide sequence around phosphorylation site of threonine 46 (S-T-T(p)-P-G) derived from Human 4E-BP1 |
抗原种属 | human |
Uniprot ID |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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