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顶部生物活性储存和溶解信息计算器

Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82)

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Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82)
产品编号 TMAC-00023
别名 p-4EBP1/2/3 (Thr45), p-4EBP1/2/3 (T45), 4EBP1/2/3 (p-Thr45), 4EBP1/2/3 (p-T45)

Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82) 是一种 Mouse 抗体，靶向 Phospho-4EBP1/2/3 (Thr45)。Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82) 可用于 WB,IP,IF。

Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82)

Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82)

Rating icon 还可以
Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82)
产品编号 TMAC-00023 别名 p-4EBP1/2/3 (Thr45), p-4EBP1/2/3 (T45), 4EBP1/2/3 (p-Thr45), 4EBP1/2/3 (p-T45)

Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82) 是一种 Mouse 抗体，靶向 Phospho-4EBP1/2/3 (Thr45)。Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82) 可用于 WB,IP,IF。

规格价格库存数量
50 μL
¥ 1,485
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82) is a Mouse antibody targeting Phospho-4EBP1/2/3 (Thr45). Anti-Phospho-4EBP1/2/3 (Thr45) Antibody (2T82) can be used in WB,IP,IF.
别名p-4EBP1/2/3 (Thr45), p-4EBP1/2/3 (T45), 4EBP1/2/3 (p-Thr45), 4EBP1/2/3 (p-T45)
交叉反应
Human,Mouse,Rat
验证活性
1. Westernblot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
2. Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A,D), untreated human 4E-BP1 transfected (B,E) and lambda protein phosphatase treated human 4E-BP1 transfected (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A,B,C) and 4E-BP1 (D,E,F).
应用
WB
IP
IF
推荐剂量
WB: 1:100-1000; IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Phospho
抗原信息
免疫原
Peptide
基因ID
1978
化学信息
分子量Theoretical: 21 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

p4EBP1/2/3 (Thr45)p4EBP1/2/3 (T45)4EBP1/2/3 (pThr45)4EBP1/2/3 (pT45)4EBP1/2/3 (phospho-Thr45)4EBP1/2/3 (phospho-T45)

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