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Anti-PCNA Antibody (8Q986)
还可以产品编号 TMAY-02249
别名 proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (8Q986) 是一种 Rabbit 抗体，靶向 PCNA。Anti-PCNA Antibody (8Q986) 可用于 WB,ELISA,IP。
Anti-PCNA Antibody (8Q986)
Anti-PCNA Antibody (8Q986)
还可以产品编号 TMAY-02249 别名 proliferating cell nuclear antigen, ATLD2
Anti-PCNA Antibody (8Q986) 是一种 Rabbit 抗体，靶向 PCNA。Anti-PCNA Antibody (8Q986) 可用于 WB,ELISA,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,694 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-PCNA Antibody (8Q986) is a Rabbit antibody targeting PCNA. Anti-PCNA Antibody (8Q986) can be used in WB,ELISA,IP. |
| 别名 | proliferating cell nuclear antigen, ATLD2 |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8Q986 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-PCNA rabbit polyclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:29 kDa. -Observed band size:35 kDa. 2. PCNA was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg MCF7 Whole Cell Lysate. -4 µL anti-PCNA rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-PCNA rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 29 kDa. -Observed band size:35 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-5 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human PCNA (rh PCNA; TMPY-02543; P12004; Met1-Ser261). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. It is central to both DNA replication and repair. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA polymerase delta and epsilon onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human PCNA Protein (TMPY-02543) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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