Anti-p53 Antibody (3K688)

Name: Anti-p53 Antibody (3K688)
Brand: TargetMol
SKU: TMAY-03857
Rating: 4.5 (1 reviews)

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产品编号 TMAY-03857

Anti-p53 Antibody (3K688) 是一种 Rabbit 抗体，靶向 p53。Anti-p53 Antibody (3K688) 可用于 WB,ELISA,IP。

Anti-p53 Antibody (3K688)

还可以

产品编号 TMAY-03857

Anti-p53 Antibody (3K688) 是一种 Rabbit 抗体，靶向 p53。Anti-p53 Antibody (3K688) 可用于 WB,ELISA,IP。

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生物活性

产品描述	Anti-p53 Antibody (3K688) is a Rabbit antibody targeting p53. Anti-p53 Antibody (3K688) can be used in WB,ELISA,IP.
Ig Type	Monoclonal Rabbit IgG
克隆号	3K688
交叉反应	Cynomolgus, Human
验证活性	1. cyno p53 was immunoprecipitated using: -Lane A:0.5 mg A431 Whole Cell Lysate -0.5 µL anti-cyno p53 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-cyno p53 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 55 kDa. -Observed band size: 55 kDa. 2. Anti-p53 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:53 kDa. -Observed band size:53 kDa
应用	WB,ELISA,IP
推荐剂量	WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.2-1 μL/mg of lysate
抗体种类	Monoclonal
宿主来源	Rabbit
构建方式	This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus p53 / TP53 (TMPY-02228; E3U906; Met1-Asp393).
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	p53, also known as Tp53, is a DNA-binding protein which belongs to the p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein levels and post-translational modification state alter in response to cellular stress (such as DNA damage, hypoxia, spindle damage). Activation of p53 begins through a number of mechanisms including phosphorylation by ATM, ATR, Chk1 and MAPKs. MDM2 is a ubiquitin ligase that binds p53 and targets p53 for proteasomal degradation. Phosphorylation, p14ARF and USP7 prevent MDM2-p53 interactions, leading to an increase in stable p53 tetramers in the cytoplasm. Further modifications such as methylation and acetylation lead to an increase in Tp53 binding to gene specific response elements. Tp53 regulates a large number of genes (>100 genes) that control a number of key tumor suppressing functions such as cell cycle arrest, DNA repair, senescence and apoptosis. Whilst the activation of p53 often leads to apoptosis, p53 inactivation facilitates tumor progression. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Defects in TP53 are a cause of esophageal cancer, Li-Fraumeni syndrome, lung cancer and adrenocortical carcinoma.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Cynomolgus p53 / TP53 protein (TMPY-02228)
抗原种属	Cynomolgus
研究领域	Apoptosis Transcription Factors and Regulators, Cancer Drug Targets, Tumor Suppressors

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.