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Anti-OTUB2 Antibody (4V813)
还可以产品编号 TMAY-02522
Anti-OTUB2 Antibody (4V813) 是一种 Rabbit 抗体，靶向 OTUB2。Anti-OTUB2 Antibody (4V813) 可用于 ELISA,ICC/IF。
Anti-OTUB2 Antibody (4V813)
Anti-OTUB2 Antibody (4V813)
还可以产品编号 TMAY-02522
Anti-OTUB2 Antibody (4V813) 是一种 Rabbit 抗体，靶向 OTUB2。Anti-OTUB2 Antibody (4V813) 可用于 ELISA,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-OTUB2 Antibody (4V813) is a Rabbit antibody targeting OTUB2. Anti-OTUB2 Antibody (4V813) can be used in ELISA,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 4V813 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of Human OTUB2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human OTUB2 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus. |
| 应用 | ELISA,ICC/IF |
| 推荐剂量 | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human OTUB2 (rh OTUB2; TMPY-02522; Q96DC9-1; Met1-His234). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human OTUB2 Protein (TMPY-02522) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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