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Anti-ORP150 Antibody (6L921)
还可以产品编号 TMAY-01846
Anti-ORP150 Antibody (6L921) 是一种 Rabbit 抗体，靶向 ORP150。Anti-ORP150 Antibody (6L921) 可用于 WB,ELISA,IHC-P,ICC/IF,IP。
Anti-ORP150 Antibody (6L921)
Anti-ORP150 Antibody (6L921)
还可以产品编号 TMAY-01846
Anti-ORP150 Antibody (6L921) 是一种 Rabbit 抗体，靶向 ORP150。Anti-ORP150 Antibody (6L921) 可用于 WB,ELISA,IHC-P,ICC/IF,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-ORP150 Antibody (6L921) is a Rabbit antibody targeting ORP150. Anti-ORP150 Antibody (6L921) can be used in WB,ELISA,IHC-P,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6L921 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human HSP12A in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to colonic gland. 2. Immunochemical staining of human HSP12A in human cirrhosis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to hepatocyte. 3. Anti-HSP12A rabbit monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:111 kDa. -Observed band size:170 kDa. 4. HSP12A was immunoprecipitated using: -Lane A:0.5 mg MCF-7 Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate. -Lane C:0.5 mg Hela Whole Cell Lysate. -2 µL anti-HSP12A rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HSP12A rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 111 kDa. -Observed band size: 111 kDa. 5. Immunofluorescence staining of ORP150 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ORP150 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm. |
| 应用 | WB,ELISA,IHC-P,ICC/IF,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ORP150 / HYOU1 / HSP12A (rh ORP150 / HYOU1 / HSP12A; TMPY-01577; NP_001124463.1; Met 695-Leu 994). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Hypoxia up-regulated protein 1, also known as 15 kDa oxygen-regulated protein, 17 kDa glucose-regulated protein, ORP-15, GRP-17, and HYOU1, is a member of the heat shock protein 7 family. Seven members from four different heat shock protein (HSP) families were identified including HYOU1 (ORP15), HSPC1 (HSP86), HSPA5 (Bip), HSPD1 (HSP6), and several isoforms of the two testis-specific HSP7 chaperones HSPA2 and HSPA1L. HYOU1 is highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. It is highly expressed in the liver and pancreas. HYOU1 is also expressed in macrophages within aortic atherosclerotic plaques and in breast cancers. HYOU1 has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. It may play a role as a molecular chaperone and participate in protein folding. Suppression of HYOU1 is associated with accelerated apoptosis. It is suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein is up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human ORP150 / HYOU1 / HSP12A protein (TMPY-01577) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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