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Anti-NUP153 Antibody (8V777)
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产品编号 TMAH-00839
别名 NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin
Anti-NUP153 Antibody (8V777) 是一种抗体，靶向 NUP153。Anti-NUP153 Antibody (8V777) 可用于 ELISA, IHC, IF。
Anti-NUP153 Antibody (8V777)
Anti-NUP153 Antibody (8V777)
还可以产品编号 TMAH-00839 别名 NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin
Anti-NUP153 Antibody (8V777) 是一种抗体，靶向 NUP153。Anti-NUP153 Antibody (8V777) 可用于 ELISA, IHC, IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-NUP153 Antibody (8V777) is an antibody targeting NUP153. Anti-NUP153 Antibody (8V777) can be used in ELISA, IHC, IF. |
| 别名 | NUP 153, Nucleoporin Nup153, Nuclear pore complex protein Nup153, 153 kDa nucleoporin |
| Ig Type | Rabbit IgG |
| 克隆号 | 8V777 |
| 交叉反应 | Human |
| 验证活性 | 1. IHC image of TMAH-00839 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of Hela Cells with TMAH-00839 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| 应用 | ELISA, IHC, IF |
| 推荐剂量 | IHC:1:50-1:200; IF:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. Nucleus membrane. Nucleus, nuclear pore complex. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with TPR, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Mediates TPR anchoring to the nuclear membrane at NPC. The repeat-containing domain may be involved in anchoring other components of the NPC to the pore membrane. Possible DNA-binding subunit of the nuclear pore complex (NPC). (Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and thereby promotes the integration of the virus in the nucleus of non-dividing cells (in vitro). (Microbial infection) Binds HIV-2 protein vpx and thereby promotes the nuclear translocation of the lentiviral genome (in vitro). |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human Nup153 |
| 抗原种属 | Human |
| 基因ID | 9972 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Tags & Cell Markers, Signal transduction |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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