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Anti-NF-kB p65 Antibody (9V807)
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产品编号 TMAB-01228
Anti-NF-kB p65 Antibody (9V807) 是一种 Mouse 抗体，靶向 NF-kB p65。Anti-NF-kB p65 Antibody (9V807) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-NF-kB p65 Antibody (9V807)
Anti-NF-kB p65 Antibody (9V807)
还可以产品编号 TMAB-01228
Anti-NF-kB p65 Antibody (9V807) 是一种 Mouse 抗体，靶向 NF-kB p65。Anti-NF-kB p65 Antibody (9V807) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-NF-kB p65 Antibody (9V807) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (9V807) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG1 |
| 克隆号 | 9V807 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Sample: RELA-Ko (Human) Cell Lysate at 30 μg Hela (Human) Cell Lysate at 30 μg Primary: Anti-NFKB p65 (RELA) (TMAB-01228) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kDa Observed band size: 65 kDa 2. 25 μg total protein per Lane of various lysates probed with NFKB p65 monoclonal antibody, unconjugated (TMAB-01228) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 3. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (NFKB p65) monoclonal Antibody, unconjugated (TMAB-01228) 1:50, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Spleen；Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01228) 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01228) 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (TMAB-01228) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC-P: 1:200-1500; IHC-Fr: 1:200-1500; ICC/IF: 1:50; IF: 1:200-1500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Nucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]. |
| 免疫原 | Recombinant Protein: human NFKB p65 Protein |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | RelA (p65),NFkB pathway,Inflammatory mediators,RelA (p65),NFkB pathway,Obesity,Host Immune Response,Host Virus Interaction,Alzheimer's disease,NFkB Pathway |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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