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Anti-NEFH Antibody (9C4) 是一种 Mouse 抗体,靶向 NEFH。Anti-NEFH Antibody (9C4) 可用于 WB,IP,IF,IHC-P。
Anti-NEFH Antibody (9C4) 是一种 Mouse 抗体,靶向 NEFH。Anti-NEFH Antibody (9C4) 可用于 WB,IP,IF,IHC-P。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,985 | 5日内发货 | |
100 μL | ¥ 2,980 | 5日内发货 |
产品描述 | Anti-NEFH Antibody (9C4) is a Mouse antibody targeting NEFH. Anti-NEFH Antibody (9C4) can be used in WB,IP,IF,IHC-P. |
别名 | NF-H, NFH, Neurofilament triplet H protein, Neurofilament heavy polypeptide, NEFH, KIAA0845, 200 kDa neurofilament protein |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of NF-H expression in rat brain (A) and mouse brain (B) tissue extracts. 2. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing membrane and cytoplasmic staining of Purkinje cells and neuropil staining in granular layer and molecular layer (B). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC-P: 1:50-500; IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Neurofilament-H (NF-H), for neurofilament heavy polypeptide, a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and Actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson's disease and Alzheimer's disease. |
偶联 | Unconjugated |
免疫原 | A neurofilament NF-H protein isolated from a cytoskeletal preparation from brain tissue homogenate of calf origin |
Uniprot ID |
分子量 | Theoretical: 200 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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