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别名 NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD
Anti-NDUFS3 Antibody (6M78) 是一种 Rabbit 抗体,靶向 NDUFS3。Anti-NDUFS3 Antibody (6M78) 可用于 ICC,IF,IHC,IP,WB。
Anti-NDUFS3 Antibody (6M78) 是一种 Rabbit 抗体,靶向 NDUFS3。Anti-NDUFS3 Antibody (6M78) 可用于 ICC,IF,IHC,IP,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
| 产品描述 | Anti-NDUFS3 Antibody (6M78) is a Rabbit antibody targeting NDUFS3. Anti-NDUFS3 Antibody (6M78) can be used in ICC,IF,IHC,IP,WB. |
| 别名 | NDUFS 3, NADH-ubiquinone oxidoreductase 30 kDa subunit, NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial, EC 1.6.99.3, EC 1.6.5.3, Complex I-30kD, CI-30kD |
| Ig Type | IgG |
| 克隆号 | 6M78 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin. 4. ICC staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining NDUFS3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ![]() ![]() ![]() ![]() ![]() |
| 应用 | ICCIFIHCIPWB |
| 推荐剂量 | WB: 1:500; IHC: 1:50; ICC: 1:50; IP: 1:10-50 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | NDUFS3 (NADH dehydrogenase ubiquinone iron-sulfur protein 3) is one of about 45 subunits comprising complex I of the oxidative phosphorylation electron transport chain. The multisubunit NADH: ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of the mitochondria. NDUFS3 is the last subunit of the seven subunits that make up the core of complex I. Through use of chaotropic agents, complex I can be separated into three different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction includes NDUFS1-7. NDUFS3 contains a highly conserved casein kinase II phosphorylation site. Mutations in the NDUFS3 gene may cause optic atrophy, Leigh syndrome and complex I deficiency. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 25 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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