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Anti-METTL1 Antibody (3N140)
还可以产品编号 TMAY-01888
Anti-METTL1 Antibody (3N140) 是一种 Mouse 抗体，靶向 METTL1。Anti-METTL1 Antibody (3N140) 可用于 WB,ELISA,ELISA(Det),IP。
Anti-METTL1 Antibody (3N140)
Anti-METTL1 Antibody (3N140)
还可以产品编号 TMAY-01888
Anti-METTL1 Antibody (3N140) 是一种 Mouse 抗体，靶向 METTL1。Anti-METTL1 Antibody (3N140) 可用于 WB,ELISA,ELISA(Det),IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-METTL1 Antibody (3N140) is a Mouse antibody targeting METTL1. Anti-METTL1 Antibody (3N140) can be used in WB,ELISA,ELISA(Det),IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 3N140 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-METTL1 mouse monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell Lysate. -Lane B: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:31 kDa. -Observed band size:31 kDa. 2. METTL1 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate. -2 µL anti-METTL1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-METTL1 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size: 34 kDa |
| 应用 | WB,ELISA,ELISA(Det),IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate; ELISA(Det): 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human METTL1 (rh METTL1; TMPY-01661; NP_005362.3; Asp 32-Gln 265). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| 研究背景 | tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human METTL1 protein (TMPY-01661) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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