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Anti-MERS-CoV Nucleocapsid Antibody (1U169) 是一种 Mouse 抗体,靶向 MERS-CoV Nucleocapsid。Anti-MERS-CoV Nucleocapsid Antibody (1U169) 可用于 WB,ELISA。
Anti-MERS-CoV Nucleocapsid Antibody (1U169) 是一种 Mouse 抗体,靶向 MERS-CoV Nucleocapsid。Anti-MERS-CoV Nucleocapsid Antibody (1U169) 可用于 WB,ELISA。
产品描述 | Anti-MERS-CoV Nucleocapsid Antibody (1U169) is a Mouse antibody targeting MERS-CoV Nucleocapsid. Anti-MERS-CoV Nucleocapsid Antibody (1U169) can be used in WB,ELISA. |
Ig Type | Monoclonal Mouse IgG1 |
克隆号 | 1U169 |
验证活性 | Anti-MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP mouse monoclonal antibody at 1:1000 -Sample:MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP -Lane A: 20ng -Lane B: 5ng -Lane C: 2ng -Secondary -Rabbit Anti-Mouse IgGF (ab)2/HRP at 1/10000 dilution.Performed under reducing conditions. |
应用 | WB,ELISA |
推荐剂量 | WB: 1:1000-1:5000 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (TMPY-03423; AFS88943.1; Met 1-Asp413). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
偶联 | Unconjugated |
免疫原 | Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (TMPY-03423) |
抗原种属 | MERS-CoV |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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