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Anti-MEK1 Antibody (1O963)
还可以产品编号 TMAY-03226
Anti-MEK1 Antibody (1O963) 是一种 Rabbit 抗体，靶向 MEK1。Anti-MEK1 Antibody (1O963) 可用于 WB,ICC/IF。
Anti-MEK1 Antibody (1O963)
Anti-MEK1 Antibody (1O963)
还可以产品编号 TMAY-03226
Anti-MEK1 Antibody (1O963) 是一种 Rabbit 抗体，靶向 MEK1。Anti-MEK1 Antibody (1O963) 可用于 WB,ICC/IF。
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偶联与修饰
抗原信息
| 产品描述 | Anti-MEK1 Antibody (1O963) is a Rabbit antibody targeting MEK1. Anti-MEK1 Antibody (1O963) can be used in WB,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 1O963 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Anti-MAP2K1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: RAW246.7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:43 kDa. -Observed band size:43 kDa. 2. Immunofluorescence staining of MEK1 in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Mouse MEK1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm. |
| 应用 | WB,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse MEK1/MAP2K1 (rM MEK1/MAP2K1; TMPY-04569; P31938; Met1-Ile393). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | MEK1, also known as MAP2K1 and MKK1, is a member of the dual-specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in the brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation, and development. Binding extracellular ligands such as growth factors, cytokines, and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause Cardiofaciocutaneous Syndrome.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Mouse MEK1/MAP2K1 Protein (TMPY-04569) |
| 抗原种属 | Mouse |
| 研究领域 | Cancer Drug Targets |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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