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Anti-MCM9 Antibody (5A998)
还可以产品编号 TMAH-00742
别名 Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9, MCMDC1DNA helicase MCM9, MCM 9, hMCM9, EC 3.6.4.12, C6orf61
Anti-MCM9 Antibody (5A998) 是一种 Mouse 抗体，靶向 MCM9。Anti-MCM9 Antibody (5A998) 可用于 ELISA, WB, IHC。
Anti-MCM9 Antibody (5A998)
Anti-MCM9 Antibody (5A998)
还可以产品编号 TMAH-00742 别名 Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9, MCMDC1DNA helicase MCM9, MCM 9, hMCM9, EC 3.6.4.12, C6orf61
Anti-MCM9 Antibody (5A998) 是一种 Mouse 抗体，靶向 MCM9。Anti-MCM9 Antibody (5A998) 可用于 ELISA, WB, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-MCM9 Antibody (5A998) is a Mouse antibody targeting MCM9. Anti-MCM9 Antibody (5A998) can be used in ELISA, WB, IHC. |
| 别名 | Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9, MCMDC1DNA helicase MCM9, MCM 9, hMCM9, EC 3.6.4.12, C6orf61 |
| Ig Type | IgG1 |
| 克隆号 | 5A998 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: SY-5Y whole cell lysate, Hela whole cell lysate,U87 whole cell lysate -All lanes: MCM9 antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 128, 73, 44, 85 kDa -Observed band size: 128 kDa -Exposure time：5min 2. IHC image of TMAH-00742 diluted at 1:100 and staining in paraffin-embedded human testes tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| 应用 | ELISA, WB, IHC |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Nucleus. Chromosome. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 纯度 | >95% |
| 研究背景 | Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand. In addition, recruits MLH1, a component of the MMR complex, to chromatin. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. Probably by regulating HR, plays a key role during gametogenesis. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human DNA helicase MCM9 Protein (1-391AA) |
| 抗原种属 | Human |
| 基因ID | 254394 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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