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Anti-LEF1 Antibody (7G348)

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Anti-LEF1 Antibody (7G348)
产品编号 TMAH-00689
别名 TCF1-alpha, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1, LEF-1

Anti-LEF1 Antibody (7G348) 是一种抗体,靶向 LEF1。Anti-LEF1 Antibody (7G348) 可用于 ELISA, IHC。

Anti-LEF1 Antibody (7G348)

Anti-LEF1 Antibody (7G348)

Rating icon 还可以
Anti-LEF1 Antibody (7G348)
产品编号 TMAH-00689 别名 TCF1-alpha, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1, LEF-1

Anti-LEF1 Antibody (7G348) 是一种抗体,靶向 LEF1。Anti-LEF1 Antibody (7G348) 可用于 ELISA, IHC。

规格价格库存数量
50 μL
¥ 1,300
5日内发货
100 μL
¥ 2,180
5日内发货
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产品介绍

生物活性
产品描述
Anti-LEF1 Antibody (7G348) is an antibody targeting LEF1. Anti-LEF1 Antibody (7G348) can be used in ELISA, IHC.
别名TCF1-alpha, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1, LEF-1
Ig Type
Rabbit IgG
克隆号
7G348
交叉反应
Human
验证活性
1. IHC image of TMAH-00689 diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.
2. IHC image of TMAH-00689 diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.
应用
推荐剂量
IHC:1:50-1:200.
抗体种类
Monoclonal
亚细胞定位Nucleus.
构建方式Recombinant Antibody
纯化方式Affinity-chromatography
性状Liquid
缓冲液Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicle morphogenesis. Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling. Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
A synthetic peptide: Human LEF1
抗原种属
Human
基因ID
Uniprot ID
研究领域
Epigenetics and Nuclear Signaling, Cancer, Developmental biology, Stem cells
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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