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Anti-LEF1 Antibody (7G348)
还可以产品编号 TMAH-00689
别名 TCF1-alpha, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1, LEF-1
Anti-LEF1 Antibody (7G348) 是一种抗体，靶向 LEF1。Anti-LEF1 Antibody (7G348) 可用于 ELISA, IHC。
Anti-LEF1 Antibody (7G348)
Anti-LEF1 Antibody (7G348)
还可以产品编号 TMAH-00689 别名 TCF1-alpha, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1, LEF-1
Anti-LEF1 Antibody (7G348) 是一种抗体，靶向 LEF1。Anti-LEF1 Antibody (7G348) 可用于 ELISA, IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,180 | 5日内发货 |
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产品介绍
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抗原信息
| 产品描述 | Anti-LEF1 Antibody (7G348) is an antibody targeting LEF1. Anti-LEF1 Antibody (7G348) can be used in ELISA, IHC. |
| 别名 | TCF1-alpha, T cell-specific transcription factor 1-alpha, Lymphoid enhancer-binding factor 1, LEF-1 |
| Ig Type | Rabbit IgG |
| 克隆号 | 7G348 |
| 交叉反应 | Human |
| 验证活性 | 1. IHC image of TMAH-00689 diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB. 2. IHC image of TMAH-00689 diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB. |
| 应用 | ELISA, IHC |
| 推荐剂量 | IHC:1:50-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicl... |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human LEF1 |
| 抗原种属 | Human |
| 基因ID | 51176 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cancer, Developmental biology, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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