Anti-LAMP1 Antibody (8V752) is a Mouse antibody targeting LAMP1. Anti-LAMP1 Antibody (8V752) can be used in ELISA,ELISA(Det),IHC-P,ICC/IF,IP.

Monoclonal Mouse IgG1

8V752

Human

1. Immunochemical staining of human LAMP1 in human placenta with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

2. Immunochemical staining of human LAMP1 in human breast carcinoma with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).

3. Confocal immunofluorescence analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human LAMP1 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.

4. LAMP1 was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-Lane B:0.5 mg Jurkat Whole Cell Lysate.

-Lane C:0.5 mg Daudi Whole Cell Lysate.

-4 µL anti-LAMP1 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-LAMP1 mouse monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 45 kDa.

-Observed band size: 113 kDa

ELISA,ELISA(Det),IHC-P,ICC/IF,IP

ELISA: 1:1000-1:2000; IHC-P: 1:50-1:200; ICC-IF: 1:20-1:100; IP: 1-4 μL/mg of lysate; ELISA(Det): 1:1000-1:10000

Monoclonal

Unconjugated

HRP

2

Recombinant Human LAMP1 protein (TMPY-01253)

Human