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Anti-KRT14 Antibody (2V710)
还可以产品编号 TMAY-00070
Anti-KRT14 Antibody (2V710) 是一种 Rabbit 抗体，靶向 KRT14。Anti-KRT14 Antibody (2V710) 可用于 WB,IHC-P,IP。
Anti-KRT14 Antibody (2V710)
Anti-KRT14 Antibody (2V710)
还可以产品编号 TMAY-00070
Anti-KRT14 Antibody (2V710) 是一种 Rabbit 抗体，靶向 KRT14。Anti-KRT14 Antibody (2V710) 可用于 WB,IHC-P,IP。
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产品介绍
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抗原信息
| 产品描述 | Anti-KRT14 Antibody (2V710) is a Rabbit antibody targeting KRT14. Anti-KRT14 Antibody (2V710) can be used in WB,IHC-P,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 2V710 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunochemical staining of human CK14 in human skin with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human CK14 in human esophageal carcinoma with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). 3. KRT14 was immunoprecipitated using: -Lane A:0.5 mg A431 Whole Cell Lysate. -2 µL anti-KRT14 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-KRT14 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 52 kDa. -Observed band size: 52 kDa. 4. Anti-Cytokeratin14 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:52 kDa. -Observed band size:52 kDa |
| 应用 | WB,IHC-P,IP |
| 推荐剂量 | WB: 1:500-1:2000; IHC-P: 1:10000-1:20000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, a synthetic peptide corresponding to the N-terminus of the human Cytokeratin 14. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in the maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and Dermatopathia Pigmentosa Reticularis. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the N-terminus of the human Cytokeratin 14. |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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