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顶部生物活性储存和溶解信息计算器

Anti-Intrinsic Factor Antibody (4J678)

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Anti-Intrinsic Factor Antibody (4J678)
产品编号 TMAY-02598
别名 TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis)

Anti-Intrinsic Factor Antibody (4J678) 是一种 Rabbit 抗体，靶向 Intrinsic Factor。Anti-Intrinsic Factor Antibody (4J678) 可用于 WB,IP。

Anti-Intrinsic Factor Antibody (4J678)
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Anti-Intrinsic Factor Antibody (4J678)

Rating icon 还可以
Anti-Intrinsic Factor Antibody (4J678)
产品编号 TMAY-02598 别名 TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis)

Anti-Intrinsic Factor Antibody (4J678) 是一种 Rabbit 抗体，靶向 Intrinsic Factor。Anti-Intrinsic Factor Antibody (4J678) 可用于 WB,IP。

规格价格库存数量
100 μL
¥ 2,279
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产品介绍

生物活性
产品描述
Anti-Intrinsic Factor Antibody (4J678) is a Rabbit antibody targeting Intrinsic Factor. Anti-Intrinsic Factor Antibody (4J678) can be used in WB,IP.
别名TCN3, INF, IFMH, IF, gastric intrinsic factor (vitamin B synthesis)
Ig Type
Rabbit IgG
克隆号
4J678
交叉反应
Human
验证活性
1. Anti-GIF rabbit monoclonal antibody at 1:500 dilution.
-Lane A: K562 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:45 kDa.
-Observed band size:45 kDa.
2. GIF was immunoprecipitated using:
-Lane A:0.5 mg K562 Whole Cell Lysate.
-Lane B:0.5 mg Jurkat Whole Cell Lysate
-0.5 µL anti-GIF rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.
-Primary antibody:
-Anti-GIF rabbit monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 45 kDa.
-Observed band size: 45 kDa
应用
WB,IP
推荐剂量
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; TMPY-03221; P27352-1; Met1-Tyr417).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human Gastric intrinsic factor / GIF Protein (TMPY-03221)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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关键词

vitamin B synthesisTCN-3TCN 3gastric intrinsic factor

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