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Anti-Intrinsic Factor Antibody (4J678)
还可以产品编号 TMAY-02598
Anti-Intrinsic Factor Antibody (4J678) 是一种 Rabbit 抗体，靶向 Intrinsic Factor。Anti-Intrinsic Factor Antibody (4J678) 可用于 WB,IP。
Anti-Intrinsic Factor Antibody (4J678)
Anti-Intrinsic Factor Antibody (4J678)
还可以产品编号 TMAY-02598
Anti-Intrinsic Factor Antibody (4J678) 是一种 Rabbit 抗体，靶向 Intrinsic Factor。Anti-Intrinsic Factor Antibody (4J678) 可用于 WB,IP。
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抗原信息
| 产品描述 | Anti-Intrinsic Factor Antibody (4J678) is a Rabbit antibody targeting Intrinsic Factor. Anti-Intrinsic Factor Antibody (4J678) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 4J678 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-GIF rabbit monoclonal antibody at 1:500 dilution. -Lane A: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:45 kDa. -Observed band size:45 kDa. 2. GIF was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate -0.5 µL anti-GIF rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-GIF rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 45 kDa. -Observed band size: 45 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Gastric intrinsic factor / GIF (rh Gastric intrinsic factor / GIF; TMPY-03221; P27352-1; Met1-Tyr417). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Gastric intrinsic factor / GIF Protein (TMPY-03221) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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