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顶部生物活性储存和溶解信息计算器

Anti-ING5 Antibody (6I821)

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Anti-ING5 Antibody (6I821)
产品编号 TMAY-02670
别名 p28ING5, inhibitor of growth family, member 5

Anti-ING5 Antibody (6I821) 是一种 Rabbit 抗体，靶向 ING5。Anti-ING5 Antibody (6I821) 可用于 WB,ELISA,IP。

Anti-ING5 Antibody (6I821)
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Anti-ING5 Antibody (6I821)

Rating icon 还可以
Anti-ING5 Antibody (6I821)
产品编号 TMAY-02670 别名 p28ING5, inhibitor of growth family, member 5

Anti-ING5 Antibody (6I821) 是一种 Rabbit 抗体，靶向 ING5。Anti-ING5 Antibody (6I821) 可用于 WB,ELISA,IP。

规格价格库存数量
100 μL
¥ 2,284
5日内发货
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产品介绍

生物活性
产品描述
Anti-ING5 Antibody (6I821) is a Rabbit antibody targeting ING5. Anti-ING5 Antibody (6I821) can be used in WB,ELISA,IP.
别名p28ING5, inhibitor of growth family, member 5
Ig Type
Rabbit IgG
克隆号
6I821
交叉反应
Human
验证活性
1. ING5 was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-ING5 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-ING5 rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 28 kDa.
-Observed band size: 32 kDa.
2. Anti-ING5 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:28 kDa.
-Observed band size:34 kDa(We are unsure as to the identity of these extra bands.)
应用
WB,ELISA,IP
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human ING5 (rh ING5; TMPY-02981; Q8WYH8-2; Glu36-Gln84).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景ING5 belongs to the ING family. It contains 1 PHD-type zinc finger and is a component of the HBO1 complex. HBO1 complex has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of PHF15, PHF16, and PHF17. ING5 also is a component of the MOZ/MORF complex which is composed at least of ING5, KAT6A, KAT6B, MEAF6, and one of BRPF1, BRD1/BRPF2, and BRPF3. It interacts with EP300 and TP53. MOZ/MORF complex has a histone H3 acetyltransferase activity. Through chromatin acetylation, ING5 may regulate DNA replication and may function as a transcriptional coactivator. It interacts with H3K4me3 and to a lesser extent with H3K4me2. ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. It can bind TP EP300/p300, a component of the histone acetyltransferase complex, suggesting its involvement in the TP53-dependent regulatory pathway.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human ING5 Protein (TMPY-02981)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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