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Anti-Influenza A Virus Hemagglutinin/HA Antibody (4B311) 是一种 Rabbit 抗体,靶向 Influenza A Virus Hemagglutinin/HA。Anti-Influenza A Virus Hemagglutinin/HA Antibody (4B311) 可用于 WB,ELISA。
Anti-Influenza A Virus Hemagglutinin/HA Antibody (4B311) 是一种 Rabbit 抗体,靶向 Influenza A Virus Hemagglutinin/HA。Anti-Influenza A Virus Hemagglutinin/HA Antibody (4B311) 可用于 WB,ELISA。
产品描述 | Anti-Influenza A Virus Hemagglutinin/HA Antibody (4B311) is a Rabbit antibody targeting Influenza A Virus Hemagglutinin/HA. Anti-Influenza A Virus Hemagglutinin/HA Antibody (4B311) can be used in WB,ELISA. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 4B311 |
验证活性 | 1. Anti-HA rabbit monoclonal antibody at 1:5000 dilution. -Sample: Recombinant Protein 10 ng -Lane 1: H1N1 (A/Brisbane/02/2018) HA -Lane 2: H1N1 (A/California/04/2009) HA -Lane 3: H1N1 (A/California/07/2009) HA -Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) HA -Lane 5: H1N1 (A/Michigan/45/2015) HA -Lane 6: H1N1 (A/Puerto Rico/8/1934) HA -Lane 7: H1N1 (A/Victoria/4897/2022) HA Protein -Lane 8: H1N1 (A/Victoria/4897/2022) HA Trimer Protein -Lane 9: H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HA -Lane 10: H1N1 (A/Wisconsin/67/2022) HA Protein -Lane 11: H1N1 (A/Wisconsin/67/2022) HA Trimer Protein -Lane 12: H3N2 (A/Brisbane/10/2007) HA -Lane 13: H3N2 (A/Cambodia/e0826360/2020) HA -Lane 14: H3N2 (A/Darwin/6/2021) HA Protein -Lane 15: H3N2 (A/Hong Kong/1/1968) HA Protein -Lane 16: H3N2 (A/Hong Kong/2671/2019) HA -Lane 17: H3N2 (A/Hong Kong/45/2019) HA -Lane 18: H3N2 (A/Hong Kong/4801/2014) HA -Lane 19: H3N2 (A/Kansas/14/2017) HA -Lane 20: H3N2 (A/Singapore/INFIMH-16-0019/2016) HA -Lane 21: H3N2 (A/Switzerland/9715293/2013) HA -Lane 22: H5N1 (A/Indonesia/5/2005) HA Protein -Lane 23: Influenza B (B/Brisbane/60/2008) HA -Lane 24: Influenza B (B/Colorado/06/2017) HA -Lane 25: Influenza B (B/Florida/4/2006) HA -Lane 26: Influenza B (B/PHUKET/3073/2013) HA -Lane 27: Influenza B (B/Washington/02/2019) HA -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. 2. Anti-HA rabbit monoclonal antibody at 1:1000 dilution. -Sample: Recombinant Protein 10 ng -Lane A: H3N2 (A/Darwin/9/2021) HA Protein -Lane B: H3N2 (A/Darwin/9/2021) HA Protein -Lane C: H3N2 (A/Darwin/6/2021) HA Protein -Lane D: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein -Lane E: Influenza B (B/Austria/1359417/2021)(Victoria lineage) HA Protein -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution -Developed using the ECL technique. -Performed under reducing conditions. 3. Anti-Influenza A Virus Hemagglutinin / HA rabbit monoclonal antibody in 1μg/mL. -Sample: Recombinant Protein 10 ng -Lane 1: Influenza A H1N1 (A/Texas/05/2009) Hemagglutinin / HA Protein -Lane 2: Influenza A H1N1 (A/Texas/05/2009) Hemagglutinin Protein (HA1 Subunit) -Lane 3: Influenza A H1N1 (A/Texas/36/1991) Hemagglutinin / HA Protein -Lane 4: Influenza A H1N1 (A/Texas/36/1991) Hemagglutinin Protein (HA1 Subunit) -Lane 5: Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin / HA Protein -Lane 6: Influenza A H3N2 (A/Texas/50/2012) Hemagglutinin Protein (HA1 Subunit) -Lane 7: Influenza A H3N2 (A/Texas/1/1977) Hemagglutinin / HA1 Protein -Lane 8: Influenza A H1N1 (A/Texas/36/1991) Hemagglutinin / HA Protein -Lane 9: Influenza A H5N1 (A/dairy cow/Texas/24-008749-002) Hemagglutinin / HA Protein -Lane 10: Influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA trimer Protein -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution -Developed using the ECL technique. -Performed under reducing conditions. |
应用 | WB,ELISA |
推荐剂量 | WB: 1:2000-1:20000; ELISA: 1:1000-1:2000 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Influenza A virus H1N1 Hemagglutinin extracellular domain. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
偶联 | Unconjugated |
免疫原 | Recombinant H1N1 HA protein |
抗原种属 | Influenza |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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